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JoVE Journal
Bioengineering

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Brug Tomoauto: En protokol til High-throughput Automatiseret Cryo-elektron Tomography

Article DOI: 10.3791/53608-v 11:33 min January 30th, 2016
January 30th, 2016

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Summary

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Click here for the English version.

Vi præsenterer en protokol om, hvordan man udnytter high-throughput cryo-elektron tomografi til at bestemme høj opløsning in situ strukturer af molekylære maskiner. Protokollen tillader store mængder data, der skal behandles, undgår fælles flaskehalse og reducerer ressource nedetid, så brugeren kan fokusere på vigtige biologiske spørgsmål.

Tags

Bioengineering , elektron cryotomography. bakterielt patogen minicelle protein sekretion injectisome molekylære maskineri high throughput billedanalyse
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