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Journal / Neuroscience / Floresan Aktif Hücre Sıralama (FACS) ve Taze ve…
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Neuroscience

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JoVE Journal Neuroscience
Floresan Aktif Hücre Sıralama (FACS) ve Taze ve Dondurulmuş Rat Beyin Dokusu gelen Fos-ifade nöronlar Gen İfadesi Analizi
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Floresan Aktif Hücre Sıralama (FACS) ve Taze ve Dondurulmuş Rat Beyin Dokusu gelen Fos-ifade nöronlar Gen İfadesi Analizi

Article DOI: 10.3791/54358-v • 08:37 min • August 27th, 2016
August 27th, 2016 •
*1, *1, 1, 2, 1
1Behavioral Neuroscience Research Branch, Intramural Research Program, NIDA, NIH, DHHS, 2Division of Rheumatology, School of Medicine, Johns Hopkins University
* These authors contributed equally

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Burada taze ve dondurulmuş hem de beyin dokusundan nöronal topluluklarından Fos-ifade moleküler değişiklikler incelemek için bir Floresan Aktif Hücre Sıralama (FACS) protokol mevcut. dondurulmuş doku kullanımı değerli hayvan deneklerin kullanımını maksimize için birden fazla seans boyunca birçok beyin bölgelerinin FACS izolasyonu sağlar.

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Neuroscience Sayı 114 hücre tipleri nöronlar glia antikorlar mRNA FACS
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