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Journal / Biochemistry / Protein Complex Affinity Capture fra Cryomilled…
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Biochemistry

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JoVE Journal Biochemistry
Protein Complex Affinity Capture fra Cryomilled pattedyrsceller
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Protein Complex Affinity Capture fra Cryomilled pattedyrsceller

Article DOI: 10.3791/54518-v • 10:37 min • December 9th, 2016
December 9th, 2016 •
1,2, 1, 1
1Laboratory of Cellular and Structural Biology, The Rockefeller University, 2Institute for Systems Genetics, Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine

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Her beskriver vi protokoller til at forstyrre pattedyrceller ved solid-state fræsning ved en kryogen temperatur, producerer en celle ekstrakt fra den resulterende celle pulver, og isolere protein komplekser af interesse ved affinitetsindfangning på antistof-koblede micron skala paramagnetiske perler.

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Biokemi affinitet capture affinitetsoprensning immunpræcipitation IP cryomilling celle forstyrrelser proteinkompleks rensning
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