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Journal / Genetics / Genetisk Manipulation af afgroedesygdomme
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Genetics

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Genetisk Manipulation af afgroedesygdomme

Article DOI: 10.3791/54522-v • 11:42 min • September 30th, 2016
September 30th, 2016 •
*1,2, *1, 3, 3, 1,2, 1,2,4
1Institute for Microbiology, Heinrich-Heine University Düsseldorf, 2Bioeconomy Science Center (BioSC), 3Department of Genetics, Institute of Applied Biosciences, Karlsruhe Institute of Technology, 4Cluster of Excellence in Plant Sciences (CEPLAS), Heinrich-Heine University Düsseldorf
* These authors contributed equally

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Vi beskriver en robust generstatning strategi til genetisk manipulere smut svampen Ustilago maydis. Denne protokol forklarer, hvordan at generere deletionsmutanter at undersøge infektion fænotyper. Det kan udvides til at modificere gener i enhver ønsket måde, fx ved tilsætning af en sekvens, der koder et fluorescerende protein-tag.

Tags

Genetik , Genteknologi sletning mutant reverse genetik mutant fænotype homolog rekombination model smut svamp plante patogen stamme verifikation
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