Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Biochemistry / Reinigung von biotinylierten…
JoVE Journal
Biochemistry
Author Produced

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Biochemistry
Reinigung von biotinylierten Zelloberflächenproteinen aus
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
Journal (Biochemistry)
Creating Highly Specific Chemically Induced Protein Dimerization Systems by Stepwise Phage Selection of a Combinatorial Single-Domain Antibody Library
A
Journal (Biochemistry)
A "Dual-Addition" Calcium Fluorescence Assay for the High-Throughput Screening of Recombinant G Protein-Coupled Receptors
 
Click here for the English version

Reinigung von biotinylierten Zelloberflächenproteinen aus

Article DOI: 10.3791/55747-v • 12:03 min • July 23rd, 2017
July 23rd, 2017 •
1, 1,2, 1
1Queensland Alliance for Agriculture & Food Innovation, The University of Queensland, 2Centre for Comparative Genomics, Murdoch University

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Eine modifizierte Dichte-Zentrifugations-Gradienten-basierte Methodik wurde verwendet, um Epithelzellen aus Rhipicephalus microplus- Darmgewebe zu isolieren. Oberflächengebundene Proteine ​​wurden biotinyliert und durch Streptavidin-Magnetperlen zur Verwendung in nachgeschalteten Anwendungen gereinigt.

Tags

Biochemie Ausgabe 125 Zelloberflächenproteine Biotinylierung Zecken Mikroplus Darm-Epithelzellen
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter