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JoVE Journal Bioengineering
Isolamento delle cellule della ganglioma della retina primaria (RGC) mediante citometria a flusso
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Isolamento delle cellule della ganglioma della retina primaria (RGC) mediante citometria a flusso

Article DOI: 10.3791/55785-v • 11:01 min • July 5th, 2017
July 5th, 2017 •
1, 1, 1, 1, 1, 2, 1,3,4, 1,2
1Department of Ophthalmology, Hamilton Eye Institute, University of Tennessee Health Science Center, 2Department of Microbiology, Immunology and Biochemistry, University of Tennessee Health Science Center, 3Department of Anatomy and Neurobiology, University of Tennessee Health Science Center, 4Department of Pharmaceutical Sciences, University of Tennessee Health Science Center

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Milioni di persone soffrono di malattie degenerative della retina che provocano cecità irreversibile. Un elemento comune di molte di queste malattie è la perdita delle cellule del ganglio retinico (RGC). Questo protocollo dettagliato descrive l'isolamento di RGC murine primari mediante selezione positiva e negativa con citometria a flusso.

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Bioingegneria Numero 125 cellule gangliari retiniche RGC citometria a flusso neurodegenerazione glaucoma invecchiamento
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