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Journal / Biochemistry / 식물 원형질에서 mRNA Interactome 캡처
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Biochemistry

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JoVE Journal Biochemistry
식물 원형질에서 mRNA Interactome 캡처
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식물 원형질에서 mRNA Interactome 캡처

Article DOI: 10.3791/56011-v • 12:29 min • July 28th, 2017
July 28th, 2017 •
1, 1, 1, 1
1Department of Biology, KU Leuven

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여기, 우리는 Arabidopsis thaliana 엽초 엽 원형체에 적용된 상호 작용 캡처 프로토콜을 제시한다. 이 방법은 생체 내 UV 가교 결합에 비판적으로 의존하며 생리 환경으로부터 식물 mRNA 결합 단백질의 분리 및 동정을 허용합니다.

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식물 생물학 이슈 125 엽초 엽 원형체 254 nm UV 공급원, 메신저 RNA 결합 단백질 올리고 (dT) 비드 메신저 리보 핵산 복합체 풀다운 SDS-PAGE,은 염색 나노 LC-MS mRNA 결합 프로테옴
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