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JoVE Journal Cancer Research
Detectie van Mitochondria membraanpotentiaal CLIC4 Knockdown-geïnduceerde HN4 cel Apoptosis om te studeren In Vitro
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Detectie van Mitochondria membraanpotentiaal CLIC4 Knockdown-geïnduceerde HN4 cel Apoptosis om te studeren In Vitro

Article DOI: 10.3791/56317-v • 13:20 min • July 17th, 2018
July 17th, 2018 •
1, 2, 2, 2, 2, 2
1School of Basic Medical Sciences, Anhui Medical University, 2Department of Orthopedics, Anhui Provincial Hospital, Anhui Medical University

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Hier presenteren we een gedetailleerd protocol voor de toepassing van rhodamine 123 CLIC4 knockdown-geïnduceerde HN4 cel apoptosis in vitrostudie te identificeren van de Mitochondriale membraanpotentiaal (MMP). Onder gemeenschappelijke fluorescentie Microscoop en confocale laser scanning fluorescentie Microscoop, werd de real-time verandering van de MMP opgenomen.

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Kankeronderzoek kwestie 137 mitochondriaal membraanpotentiaal rhodamine 123 confocale laser scanning fluorescentie Microscoop chloride intracellulaire channel 4 apoptosis hoofd en nek squamous carcinoom
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