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Isolatie van Intact, hele muis borstklieren p.a. van extracellulaire Matrix expressie en klier morfologie
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Isolatie van Intact, hele muis borstklieren p.a. van extracellulaire Matrix expressie en klier morfologie

Article DOI: 10.3791/56512-v • 12:49 min • October 30th, 2017
October 30th, 2017 •
1, 2, 2
1Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, 2Department of Biomedical Sciences, GA-PCOM

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Hier presenteren we een protocol voor de isolatie van geheel, intact muis borstklieren om te onderzoeken van de extracellulaire matrix (ECM) expressie en ductaal morfologie. Muis #4 abdominale klieren werden gehaald uit de 8-10 week oude vrouwelijke nullipaar muizen, vast in neutraal gebufferde formaline, gesegmenteerd en gekleurd met behulp van immunohistochemistry voor ECM eiwitten.

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Fysiologie kwestie 128 extracellulaire matrix borstklieren leidingen immunohistochemistry
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