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Journal / Immunology and Infection / Deteção e remoção de contaminação de Nuclease…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Deteção e remoção de contaminação de Nuclease durante a purificação do protótipo recombinante vírus espumoso Integrase
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Deteção e remoção de contaminação de Nuclease durante a purificação do protótipo recombinante vírus espumoso Integrase

Article DOI: 10.3791/56605-v • 07:50 min • December 8th, 2017
December 8th, 2017 •
1, 1, 1, 1
1Department of Cancer Biology and Genetics, The Ohio State University College of Medicine

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Proteína de integrase espumosa vírus recombinante protótipo é frequentemente contaminada com uma nuclease bacteriana durante a purificação. Este método identifica a contaminação da nuclease e remove-lo a preparação final da enzima.

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Imunologia edição 130 retrovírus vírus espumoso protótipo Integrase Nuclease purificação de proteínas
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