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Un test Flow Cytometry pour mesurer le potentiel de Membrane Mitochondrial dans les Myocytes cardiaques après l’hypoxie/réoxygénation
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Un test Flow Cytometry pour mesurer le potentiel de Membrane Mitochondrial dans les Myocytes cardiaques après l’hypoxie/réoxygénation

Article DOI: 10.3791/57725-v • 07:14 min • July 13th, 2018
July 13th, 2018 •
1, 1, 2, 2
1State Key Laboratory of Cardiovascular Disease, Department of Cardiology, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College, 2State Key Laboratory of Cardiovascular Disease, Fuwai Hospital, National Center for Cardiovascular Diseases, Chinese Academy of Medical Sciences and Peking Union Medical College

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Nous présentons ici un protocole qui utilise JC-1 colorant pour évaluer le potentiel de la membrane mitochondriale des cellules après avoir été exposés à une hypoxie/réoxygénation avec ou sans agent de protection.

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La perméabilité mitochondriale comportement numéro 137 transition pore potentiel de membrane mitochondrial JC-1 dosage lésion de reperfusion myocytes cardiaques cytométrie en flux
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