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Journal / Neuroscience / FM kleurstof fietsen in de synaps: vergelijken…
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JoVE Journal Neuroscience
FM kleurstof fietsen in de synaps
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FM kleurstof fietsen in de synaps: vergelijken van hoge kalium depolarisatie, elektrische en Channelrhodopsin stimulatie

Article DOI: 10.3791/57765-v • 08:31 min • May 24th, 2018
May 24th, 2018 •
1, 2
1Department of Biological Sciences, Vanderbilt University, 2Departments of Biological Sciences, Pharmacology, Cell and Developmental Biology, Kennedy Center for Research on Human Development, Vanderbilt University and Medical Center

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Synaptic Vesikel (SV) fietsen is het mechanisme van de kern van de intercellulaire communicatie in neuronale synapsen. FM kleurstof opname- en release zijn het belangrijkste middel van kwantitatief onderzoek SV endo- en exocytose. Hier, vergelijken wij alle stimulatie methoden om te rijden FM1-43 fietsen in de Drosophila motorische eindplaat (NMJ) model synaps.

Tags

Neurowetenschappen kwestie 135 Drosophila motorische eindplaat FM1-43 electrofysiologie photoconversion transmissie-elektronenmicroscopie
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