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Journal / Biochemistry / Spektrofotometrisk bestämning av Phycobiliprotein …
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JoVE Journal Biochemistry
Spektrofotometrisk bestämning av Phycobiliprotein innehåll i Cyanobakterien Synechocystis
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Spektrofotometrisk bestämning av Phycobiliprotein innehåll i Cyanobakterien Synechocystis

Article DOI: 10.3791/58076-v • 08:08 min • September 11th, 2018
September 11th, 2018 •
1, 1,2, 3, 1
1Department of Adaptive Biotechnologies, Global Change Research Institute, Czech Academy of Sciences, 2Department of Plant Physiology, Faculty of Science, Masaryk University, 3Laboratory of Intracellular Regulation, Institute of Plant Physiology, Russian Academy of Sciences

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Här presenterar vi ett protokoll för att kvantitativt bestämma phycobiliprotein innehåll i Cyanobakterien Synechocystis med en spektrofotometrisk metod. Extraktionsmetod tillämpades också till andra cyanobakterier och alger stammar; på grund av variationer i pigment Absorptionsspektra är det dock nödvändigt att testa spektrofotometrisk ekvationerna för varje stam individuellt.

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Biokemi fråga 139 Phycobilisomes phycobilins phycocyanobilins phycocyanin allophycocyanin pigment ljus skörd cyanobakterier spektrofotometri alger protein utvinning
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