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JoVE Journal Developmental Biology
Visualiseren van Drosophila been Motor Neuron axonen via de volwassen cuticula
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Visualiseren van Drosophila been Motor Neuron axonen via de volwassen cuticula

Article DOI: 10.3791/58365-v • 08:33 min • October 30th, 2018
October 30th, 2018 •
1, 2, 3, 2, 1
1Institut de Génomique Fonctionnelle de Lyon, ENS de Lyon, CNRS, 2Departments of Biochemistry and Molecular Biophysics, and Neuroscience, Mortimer B. Zuckerman Mind Brain Behavior Institute, Columbia University, 3Neuroscience Program, NYU School of Medicine

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Hier beschrijven we een protocol om te visualiseren de axonale richt met een TL proteïne in volwassen benen van Drosophila door fixatie, montage, imaging en na imaging stappen.

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Ontwikkelingsbiologie kwestie 140 Drosophila been motorische neuronen confocal microscopie axonen imaging groen fluorescente proteïne (GFP) cuticula
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