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Journal / Immunology and Infection / Intubação oral de Zebrafish adulto: um modelo…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Intubação oral de Zebrafish adulto
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Intubação oral de Zebrafish adulto: um modelo para avaliar a absorção Intestinal de compostos bioativos

Article DOI: 10.3791/58366-v • 04:33 min • September 27th, 2018
September 27th, 2018 •
1,2, 1,2, 1,2
1Institute of Biotechnology and Biomedicine (IBB-UAB), Universitat Autònoma de Barcelona, 2Department of Cell Biology, Animal Physiology and Immunology, Universitat Autònoma de Barcelona

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O protocolo descreve intubação zebrafish adulto com um biológico; em seguida, dissecando e preparar o intestino para citometria, microscopia confocal e qPCR. Este método permite a administração de compostos bioativos para monitorar a absorção intestinal e o estímulo imune local evocado. É relevante para a dinâmica intestinal da profilaxia oral de teste.

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Imunologia e infecção edição 139 Zebrafish intubação oral intestino imunidade mucosal citometria imagem latente confocal qPCR
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