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Journal / Neuroscience / Recuperación de biocitina y reconstrucciones 3D…
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Neuroscience

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JoVE Journal Neuroscience
Recuperación de biocitina y reconstrucciones 3D de interneuronas lleno Hippocampal CA2
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Journal (Neuroscience)
Single-cell RNA Sequencing of Fluorescently Labeled Mouse Neurons Using Manual Sorting and Double In Vitro Transcription with Absolute Counts Sequencing (DIVA-Seq)
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Recuperación de biocitina y reconstrucciones 3D de interneuronas lleno Hippocampal CA2

Article DOI: 10.3791/58592-v • 11:21 min • November 20th, 2018
November 20th, 2018 •
*1, *1, 1
1Department of Pharmacology, University College London
* These authors contributed equally

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El protocolo que aquí describe el análisis de la inmunofluorescencia, recuperación de biocitina y reconstrucciones de alta calidad de interneuronas de CA2 hipocampales siguiendo los registros electrofisiológicos intracelulares in vitro, permitiendo neuronal caracterización y finalmente fina anatomía neuronal para ser estudiado.

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Neurociencia número 141 Immunohistochemistry protocolo de la inmunofluorescencia protocolo HRP reconstrucciones neuronales Neurolucida Camera Lucida anatomía neuronal dendritas axón
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