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Detectie van Tilapia Lake Virus met behulp van conventionele rechts-PCR en SYBR groene RT-qPCR
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Detectie van Tilapia Lake Virus met behulp van conventionele rechts-PCR en SYBR groene RT-qPCR

Article DOI: 10.3791/58596-v • 10:55 min • November 10th, 2018
November 10th, 2018 •
1, 2, 2
1Institute of Veterinary Bacteriology Vetsuisse Faculty, University of Bern, 2Department of Veterinary Microbiology and Immunology and Center for Advanced Studies for Agriculture and Food, Kasetsart University Institute for Advanced Studies, Kasetsart University

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Dit protocol diagnose Tilapia Lake Virus (TiLV) in tilapia weefsels met behulp van de RT-PCR methodes. De hele methode is beschreven van weefsel dissectie totaal RNA extractie, gevolgd door de cDNA synthese en opsporing van TiLV door conventionele PCR of kwantitatieve PCR dsDNA bindend een bindende fluorescerende kleurstof gebruiken.

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Milieuwetenschappen kwestie 141 Tilapia lake virus tilapia diagnose RT-PCR RT-qPCR orthomyxo-achtig virus
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