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Journal / Neuroscience / 荧光标记小鼠神经元的单细胞 RNA 序列的人工分选和双体外转录与绝对计数测序…
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JoVE Journal Neuroscience
荧光标记小鼠神经元的单细胞 RNA 序列的人工分选和双体外转录与绝对计数测序 (天后-Seq)
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荧光标记小鼠神经元的单细胞 RNA 序列的人工分选和双体外转录与绝对计数测序 (天后-Seq)

Article DOI: 10.3791/58690-v • 07:49 min • October 26th, 2018
October 26th, 2018 •
1, 1
1Cold Spring Harbor Laboratory

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本协议描述了将单个荧光标记神经元与体外转录的 mRNA 扩增分离以进行高深度单细胞 RNA 测序的人工分拣程序。

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神经科学 问题 140 手动排序 神经元 单细胞 RNA 测序 天后序列,体外转录 线性扩增 唯一分子标识符 (海)
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