JoVE Journal
Bioengineering
Bioengineering
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Summary
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我们提出了荧光测定,以证明Lucifer黄色(LY)是一个强大的标记,以确定hCMEC/D3细胞单层的明显准细胞渗透性,人类血脑屏障的体外模型。我们使用这种测定来确定培养的hCMEC/D3细胞中汇体单层形成的动力学。