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Biochemistry

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JoVE Journal Biochemistry
Fluorescerende visualisering av mango-Tagged RNA i polyakrylamid gels via en Poststaining metode
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Fluorescerende visualisering av mango-Tagged RNA i polyakrylamid gels via en Poststaining metode

Article DOI: 10.3791/59112-v • 06:06 min • June 21st, 2019
June 21st, 2019 •
*1, *1, 1
1Department of Molecular Biology and Biochemistry, Simon Fraser University
* These authors contributed equally

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Her presenterer vi en følsom, rask, og diskriminerende post-gel Fargemetode til bilde RNAs tagget med RNA mango aptamers I, II, III, eller IV, ved hjelp av enten native eller denaturering polyakrylamid gel elektroforese (PAGE) gels. Etter å ha kjørt standard PAGE gels, kan mango-Tagged RNA enkelt farget med TO1-biotin og deretter analyseres ved hjelp av allment tilgjengelige fluorescens lesere.

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Biokjemi RNA mango fluorescens denaturering gels Native gels gel farging deteksjon metode PAGE
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