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Journal / Neuroscience / Umwandlung von vom Menschen induktiven…
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Neuroscience

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JoVE Journal Neuroscience
Umwandlung von vom Menschen induktiven Pluripotent-Stammzellen (iPSCs) in funktionale Wirbelsäulen-und Krankheitsanleitungsneuronen mit PiggyBac-Vektoren
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Umwandlung von vom Menschen induktiven Pluripotent-Stammzellen (iPSCs) in funktionale Wirbelsäulen-und Krankheitsanleitungsneuronen mit PiggyBac-Vektoren

Article DOI: 10.3791/59321-v • 07:33 min • May 1st, 2019
May 1st, 2019 •
1, 2, 2, 2, 3, 2,4, 2,4, 1,2
1Department of Biology and Biotechnology Charles Darwin, Sapienza University of Rome, Italy, 2Center for Life Nano Science, Istituto Italiano di Tecnologia, Italy, 3Laboratory of Stem Cell biology and Molecular Embryology, The Rockefeller University, USA, 4Department of Physiology and Pharmacology, Sapienza University of Rome, Italy

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Dieses Protokoll ermöglicht eine schnelle und effiziente Umwandlung von induzierten pluripotenten Stammzellen in motorische Neuronen mit einer Wirbelsäulen-oder Schädelidentität, indem es die Transkriptionsfaktoren von induzierbaren PiggyBac-Vektoren ausführt.

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Neurowissenschaften Ausgabe 147 induzierte pluripotente Stammzellen motorische Neuronen piggyBac Differenzierung Transkriptionsfaktoren Phox2a Isl1 Ngn2 Lhx3
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