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JoVE Journal
Biochemistry

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通过亲和色谱与西布洛特或质谱结合的亲致色谱,识别磷酸磷酸磷酸磷酸醇或磷酸蛋白
 
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通过亲和色谱与西布洛特或质谱结合的亲致色谱,识别磷酸磷酸磷酸磷酸醇或磷酸蛋白

Article DOI: 10.3791/59865-v 08:07 min July 26th, 2019
July 26th, 2019

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Summary

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该协议侧重于识别与磷酸肌醇或磷酸磷酸剂结合的蛋白质。它使用亲和色谱与生物异化异酸醇磷酸盐或磷酸磷酸酯,通过链球菌固定到胶质或磁珠。磷酸磷酸磷酸磷酸磷酸磷酸二醇结合蛋白通过西方印迹或质谱法鉴定。

Tags

本月在JoVE 第149期 肌醇磷酸盐 磷脂酰二醇 磷脂苷酸 蛋白质相互作用,锥形瘤 代谢物 质谱 西印迹 亲和色谱
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