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JoVE Journal Cancer Research
Fremstilling af mitokondrier fra æggestokkene Cancer væv og kontrol ovarie væv til kvantitativ proteomics analyse
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Fremstilling af mitokondrier fra æggestokkene Cancer væv og kontrol ovarie væv til kvantitativ proteomics analyse

Article DOI: 10.3791/60435-v • 11:51 min • November 18th, 2019
November 18th, 2019 •
1,2,3,4, 5, 1,2,3, 1,2,3, 1,2,3
1Key Laboratory of Cancer Proteomics of Chinese Ministry of Health, Xiangya Hospital, Central South University, 2Hunan Engineering Laboratory for Structural Biology and Drug Design, Xiangya Hospital, Central South University, 3State Local Joint Engineering Laboratory for Anticancer Drugs, Xiangya Hospital, Central South University, 4National Clinical Research Center for Geriatric Disorders, Xiangya Hospital, Central South University, 5Department of Obstetrics and Gynecology, Xiangya Hospital, Central South University

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Denne artikel præsenterer en protokol med differentialhastighed centrifugering i kombination med tæthed gradient centrifugering at adskille mitokondrier fra humane æggestokkene Cancer væv og kontrol ovarie væv til kvantitativ proteomics analyse, resulterer i en høj kvalitet mitokondrie prøve og høj gennemløb og høj reproducerbarhed kvantitativ proteomics analyse af en menneskelig ovariecancer mitokondrie proteome.

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Kræftforskning kræft i æggestokkene mitokondrier differentialhastighed centrifugering tæthed gradient centrifugering isobarisk tag for relative og absolutte kvantificering (itraq) mærkning stærk kation udveksling (SCX) væskekromatografi (LC) tandem massespektrometri (MS/MS) mitokondrie proteom
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