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Culture de la petite colonie Variante de Pseudomonas aeruginosa et Quantitation de son Alginate
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Culture de la petite colonie Variante de Pseudomonas aeruginosa et Quantitation de son Alginate

Article DOI: 10.3791/60466-v • 09:06 min • February 22nd, 2020
February 22nd, 2020 •
*1, *2, 1,2,3
1Department of Biomedical Sciences, Joan C. Edwards School of Medicine, Marshall University, 2Progenesis Technologies LLC, Robert C. Byrd Biotechnology Science Center, 3Department of Pediatrics, Joan C. Edwards School of Medicine, Marshall University
* These authors contributed equally

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Ici, nous décrivons une condition de croissance à la culture de la variante petite colonie de Pseudomonas aeruginosa. Nous décrivons également deux méthodes séparées pour la détection et la quantitation de l’alginate d’exopolysaccharide produit par P. aeruginosa utilisant un test traditionnel de carbazole d’acide uronique et un anticorps monoclonal alginate-spécifique (mAb) basé ELISA.

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Immunologie et infection numéro 156 Pseudomonas aeruginosa fibrose kystique (CF) petite variante de colonie (SCV) biosynthèse de pyrimidine croissance mucoidy alginate analyse de carbazole d’acide uronique mAb alginate-spécifique ELISA
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