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Journal / Developmental Biology / Laser Capture Microdissection av mus embryonale…
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Developmental Biology

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JoVE Journal Developmental Biology
Laser Capture Microdissection av mus embryonale brusk og bein for Gene Expression analyse
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Laser Capture Microdissection av mus embryonale brusk og bein for Gene Expression analyse

Article DOI: 10.3791/60503-v • 09:20 min • December 18th, 2019
December 18th, 2019 •
1, 1, 1,2, 1, 1
1Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, 2Icahn Institute for Data Science and Genomic Technology, Icahn School of Medicine at Mount Sinai

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Denne protokollen beskriver laser fangst microdissection for isolering av brusk og bein fra friske frosne deler av musen embryo. Brusk og bein kan raskt visualisere av cresyl fiolett farging og samlet nettopp for å gi høy kvalitet RNA for transcriptomic analyse.

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Utviklingsbiologi laser fangst microdissection Horst ' s brusk søvnapnéskinne bein cresyl fiolett RNA isolasjon RNA sekvensering
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