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Journal / Genetics / Bruk av pyrimidinanalog, 5-jod-2'-deoksyuridin…
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Genetics

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JoVE Journal Genetics
Bruk av pyrimidinanalog, 5-jod-2'-deoksyuridin (IdU) med cellesyklusmarkører for å etablere cellesyklusfaser i en massecytometriplattform
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Bruk av pyrimidinanalog, 5-jod-2'-deoksyuridin (IdU) med cellesyklusmarkører for å etablere cellesyklusfaser i en massecytometriplattform

Article DOI: 10.3791/60556-v • 08:37 min • October 22nd, 2021
October 22nd, 2021 •
1, 1
1Ohio State Comprehensive Cancer Center, Division of Hematology, The Ohio State University

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Denne protokollen tilpasser cellesyklusmålinger for bruk i en massecytometriplattform. Med multiparameteregenskapene til massecytometri tillater direkte måling av jodinkorporering identifisering av celler i S-fase mens intracellulære syklusmarkører muliggjør karakterisering av hver cellesyklustilstand under en rekke eksperimentelle forhold.

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Retraksjon utgave 176 massecytometri cellesyklus jod-deoksyuridin bestråling DNA-skaderespons cellelinje immunceller
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