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JoVE Journal Chemistry
Kvantificering af metal udvaskning i immobiliseret Metalaffinitets kromatografi
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Kvantificering af metal udvaskning i immobiliseret Metalaffinitets kromatografi

Article DOI: 10.3791/60690-v • 05:35 min • January 17th, 2020
January 17th, 2020 •
1, 1, 2, 1
1Department of Chemistry and Biochemistry, James Madison University, 2Biophysics Graduate Program, Ohio State University

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Vi præsenterer en analyse for nem kvantificering af metaller introduceret til prøver tilberedt ved hjælp af immobiliseret metalaffinitets kromatografi. Metoden bruger hydroxynaphthol blå som den kolorimetriske metal indikator og et UV-Vis Spektrofotometer som detektoren.

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Kemi immobiliseret metal affinitets kromatografi hydroxynaphthol blå HNB ni-NTA metalforurening hans-tag
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