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JoVE Journal
Developmental Biology

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研究对牙髓寄生虫信号反应的神经质生长的共同培养方法
 
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研究对牙髓寄生虫信号反应的神经质生长的共同培养方法

Article DOI: 10.3791/60809-v 08:06 min February 14th, 2020
February 14th, 2020

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Summary

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Click here for the English version.

我们描述了牙髓(DP)原细胞的分离、分散和电镀,这些细胞在覆盖的透井过滤器上培养三叉(TG)神经元。DP细胞的细胞反应可以通过免疫荧光或RNA/蛋白质分析进行分析。神经元标记物与共聚焦显微镜的免疫荧光允许分析神经质外生长反应。

Tags

发育生物学,第156期,神经科学,发育生物学,牙齿,内侧,三叉神经结,共培养方法
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