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Biochemistry

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JoVE Journal Biochemistry
Acyl-PEGyl Exchange Gel Shift Assay voor kwantitatieve bepaling van palmitoylation van Brain Membrane Eiwitten
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
Journal (Biochemistry)
Evaluation of Protein–Protein Interactions using an On-Membrane Digestion Technique
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography (Video) | JoVE
Journal (Biochemistry)
High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography (Video) | JoVE
 
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Acyl-PEGyl Exchange Gel Shift Assay voor kwantitatieve bepaling van palmitoylation van Brain Membrane Eiwitten

Article DOI: 10.3791/61018-v • 08:28 min • March 29th, 2020
March 29th, 2020 •
1, 1
1Department of Pharmacology, UC Davis School of Medicine

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Palmitoylation houdt de integratie in van een 16-koolstof palmitaat moiety aan cysteïneresiduen van doelproteïnen op een omkeerbare manier. Hier beschrijven we een biochemische benadering, de acyl-PEGyl exchange gel shift (APEGS) test, om de palmitoylation toestand van een eiwit van belang in muis hersenen lysaten te onderzoeken.

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Biochemie Nummer 157 hersenen synaps palmitoylation AMPA receptor SynDIG1 SynDIG4 Prrt1 Prrt2 APEGS test
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