JoVE Journal
Immunology and Infection
Immunology and Infection
A subscription to JoVE is required to view this content. Sign in or start your free trial.
Chapters
Summary
We describe a protocol for a three-dimensional co-culture model of infected airways, using CFBE41o- cells, THP-1 macrophages, and Pseudomonas aeruginosa, established at the air-liquid interface. This model provides a new platform to simultaneously test antibiotic efficacy, epithelial barrier function, and inflammatory markers.