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Rekonstruktion af enkeltcellede medfødte fluorescenssignaturer af konfokal mikroskopi
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Rekonstruktion af enkeltcellede medfødte fluorescenssignaturer af konfokal mikroskopi

Article DOI: 10.3791/61120-v • 07:29 min • May 27th, 2020
May 27th, 2020 •
*1, *2, 1, 2,3, *2,3
1Graduate School of Life and Environmental Sciences, University of Tsukuba, 2Faculty of Life and Environmental Sciences, University of Tsukuba, 3Microbiology Research Center for Sustainability, University of Tsukuba
* These authors contributed equally

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Her præsenteres en protokol til optisk udvinding og katalogisering af medfødte cellulære fluorescenssignaturer (dvs. cellulær autofluorescence) fra hver enkelt levende celle fordelt i et tredimensionelt rum. Denne metode er velegnet til at studere den medfødte fluorescenssignatur af forskellige biologiske systemer ved en enkeltcellet opløsning, herunder celler fra bakterier, svampe, gær, planter og dyr.

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Biologi Udgave 159 konfokal mikroskopi spektroskopi autofluorescence enkeltcellet analyse medfødt fluorescens konfokal refleksion mikroskopi konfokal mikrospektroskopi mikrobiologi tag-fri analyse minimalt invasiv analyse
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