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Minimere hypoksi i Hippocampal skiver fra voksne og aldrende mus
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Minimere hypoksi i Hippocampal skiver fra voksne og aldrende mus

Article DOI: 10.3791/61377-v • 08:58 min • July 2nd, 2020
July 2nd, 2020 •
1
1Department of Biology, Neurobiology, and Bio-X, Stanford University

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Dette er en protokoll for akutt skive forberedelse fra voksen og aldrende mus hippocampi som utnytter transcardial perfusjon og skive skjæring med iskald NMDG-aCSF for å redusere hypoksisk skade på vevet. De resulterende skivene holder seg friske over mange timer, og er egnet for langsiktig patch-clamp og feltopptak.

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Nevrovitenskap Utgave 161 Hippocampal skiver voksen aldring hypoksi ATP NMDG CA1 patch-clamp feltopptak
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