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Journal / Immunology and Infection / Neisseria meningitidis Infecção de Células-Tronco …
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Immunology and Infection

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JoVE Journal Immunology and Infection
Neisseria meningitidis Infecção de Células-Tronco Pluripotentes Induzidas Derivadas do Endotélio Cerebral
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Neisseria meningitidis Infecção de Células-Tronco Pluripotentes Induzidas Derivadas do Endotélio Cerebral

Article DOI: 10.3791/61400-v • 10:12 min • July 14th, 2020
July 14th, 2020 •
1, 2, 1, 1,2
1Institute for Hygiene and Microbiology, University of Würzburg, 2Department of Biological Sciences, University of Alabama

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O protocolo aqui descrito destaca as principais etapas na diferenciação de células endoteliais cerebrais derivadas de células-tronco pluripotentes induzidas, na preparação de Neisseria meningitidis para infecção e na coleta de amostras para outras análises moleculares.

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Neisseria Meningitidis Meningite Meningocócica Células Endoteliais Cerebrais Interações Hospedeiro-Patógeno Sistemas Modelo In Vivo Modelo Humano Junções Apertadas Resistência Elétrica Transendotelial (TEER) Modelos In Vitro CEBs Primárias CEBs Imortalizadas Células-Tronco Pluripotentes Induzidas (iPSCs) CEBs derivadas de IPSC (iPSC-BECs) Destruição de Barreiras Ativação Imune Inata Interação Bacteriana
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