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Journal / Biochemistry / Messung der Nukleotidbindung an intakte,…
JoVE Journal
Biochemistry

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JoVE Journal Biochemistry
Messung der Nukleotidbindung an intakte, funktionelle Membranproteine in Echtzeit
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Messung der Nukleotidbindung an intakte, funktionelle Membranproteine in Echtzeit

Article DOI: 10.3791/61401-v • 08:33 min • March 11th, 2021
March 11th, 2021 •
1, 1, 1,2
1Department of Physiology, Anatomy and Genetics, University of Oxford, 2Department of Chemistry and Neuroscience Program, Trinity College

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Dieses Protokoll stellt eine Methode zur Messung der Adenin-Nukleotid-Bindung an Rezeptoren in Echtzeit in einer zellulären Umgebung vor. Die Bindung wird als Förster-Resonanzenergietransfer (FRET) zwischen Trinitrophenylnukleotidderivaten und Proteinen, die mit einer nicht-kanonischen, fluoreszierenden Aminosäure markiert sind, gemessen.

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Diesen Monat in JoVE Ausgabe 169 Ligandenbindung Membranprotein Ionenkanal Nukleotid Methode Patch-Clamp Fluoreszenz Spektren Rezeptor
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