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JoVE Journal Environment
Detektion av Phytophthora capsici i Irrigation Water med hjälp av Loop-medierad Isotermisk Förstärkning
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Detektion av Phytophthora capsici i Irrigation Water med hjälp av Loop-medierad Isotermisk Förstärkning

Article DOI: 10.3791/61478-v • 07:25 min • June 25th, 2020
June 25th, 2020 •
1, 1, 2, 3, 4, 1
1Department of Plant Pathology, Coastal Plain Experiment Station, University of Georgia, 2University of Georgia Cooperative Extension, 3Department of Plant Pathology, Tropical Research & Education Center, University of Florida, 4Department of Agricultural and Environmental Sciences, Otis L. Floyd Nursery Research Center, Tennessee State University

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Vi utvecklat en metod för att upptäcka Phytophthora capsici zoosporer i vattenkällor med hjälp av ett filter papper DNA extraktion metod tillsammans med en loop-medierad isotermisk förstärkning (LAMP) analys som kan analyseras i fält eller i labbet.

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Denna månad i JoVE Phytophthora capsici Bevattningsvatten Zoospore upptäckt LAMP analys Filter papper Snabb upptäckt DNA-extraktion In-field diagnos
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