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JoVE Journal
Biology

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分离和富集人肺上皮祖细胞用于类器官培养
 
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分离和富集人肺上皮祖细胞用于类器官培养

Article DOI: 10.3791/61541-v 11:49 min July 21st, 2020
July 21st, 2020

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Summary

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Click here for the English version.

本文提供了组织解离和细胞分馏方法的详细方法,允许从人肺的近端和远端区域富集活的上皮细胞。本文将这些方法应用于通过使用3D类器官培养模型对肺上皮祖细胞进行功能分析。

Tags

生物学,第161期,肺,上皮,类器官培养,疾病建模,肺泡II型细胞,FACS
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