Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Cancer Research / Kernisolatie van vers bevroren hersentumoren voor …
JoVE Journal
Cancer Research

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Cancer Research
Kernisolatie van vers bevroren hersentumoren voor single-nucleus RNA-seq en ATAC-seq
A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies
Journal (Cancer Research)
A Syngeneic Mouse Model of Metastatic Renal Cell Carcinoma for Quantitative and Longitudinal Assessment of Preclinical Therapies
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Journal (Cancer Research)
Yeast As a Chassis for Developing Functional Assays to Study Human P53
 
Click here for the English version

Kernisolatie van vers bevroren hersentumoren voor single-nucleus RNA-seq en ATAC-seq

Article DOI: 10.3791/61542-v • 06:22 min • August 25th, 2020
August 25th, 2020 •
1, 2, 1, 1,3, 4, 2, 1
1Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, 2Bioinformatics and Omics Data Analytics, German Cancer Research Center (DKFZ), 3Clinical Cooperation Unit Neurooncology, German Cancer Research Center (DKFZ), 4Division of Experimental Neurosurgery, Department of Neurosurgery, University of Heidelberg, INF 400, Heidelberg, Germany

Chapters

Summary
Automatic Translation

Please note that all translations are automatically generated.

Click here for the English version.

Intratumorale heterogeniteit is een inherent kenmerk van tumoren, waaronder gliomen. We ontwikkelden een eenvoudig en efficiënt protocol dat een combinatie van buffers en gradiëntcentrifugatie gebruikt om enkele kernen te isoleren uit vers bevroren glioomweefsels voor single nucleus RNA en ATAC-sequencingstudies.

Tags

Kankeronderzoek Kernisolatie snRNA-seq snATAC-seq 10x Genomics Glioma
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter