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Journal / Genetics / Genetischer Variantennachweis im CALR-Gen mittels …
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JoVE Journal Genetics
Genetischer Variantennachweis im CALR-Gen mittels hochauflösender Schmelzanalyse
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Genetischer Variantennachweis im CALR-Gen mittels hochauflösender Schmelzanalyse

Article DOI: 10.3791/61642-v • 08:46 min • August 26th, 2020
August 26th, 2020 •
1,2, 1, 1,3, 1, 1, 1, 1
1Department of Hematology, University Medical Centre Ljubljana, 2Clinical Institute for Genomic Medicine, University Medical Centre Ljubljana, 3Faculty of Pharmacy, University of Ljubljana

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Die hochauflösende Schmelzanalyse (HRM) ist eine empfindliche und schnelle Lösung für den genetischen Variantennachweis. Es hängt von Sequenzunterschieden ab, die dazu führen, dass Heteroduplexe die Form der Schmelzkurve verändern. Durch Kämmen von HRM und Agarosegelelektrophorese können verschiedene Arten von genetischen Varianten wie Indels identifiziert werden.

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Genetics High resolution melting analysis fluorescence-based quantitative real-time polymerase chain reaction genetic variant pre-cast agarose gel electrophoresis indel somatic mutation heteroduplex scanning CALR
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