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Journal / Immunology and Infection / Konjunktivval kommensal isolering och…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Konjunktivval kommensal isolering och identifiering hos möss
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Journal (Immunology and Infection)
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
Journal (Immunology and Infection)
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Konjunktivval kommensal isolering och identifiering hos möss

Article DOI: 10.3791/61672-v • 07:52 min • May 1st, 2021
May 1st, 2021 •
1, 1, 1, 2,3, 2,3, 1
1Department of Medicine, Division of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, 2Massachusetts Host-Microbiome Center, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School, 3Clinical Microbiology Laboratory, Department of Pathology, Brigham and Women's Hospital, Harvard Medical School

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Presenteras här är ett protokoll för isolering och förstärkning av aeroba och facultative anaeroba mus konjunktival kommensala bakterier med hjälp av en unik öga svabba och kultur-baserade anrikning steg med efterföljande identifiering av mikrobiologiska baserade metoder och MALDI-TOF massa spektrometri.

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Denna månad i JoVE utgåva 171 mus okulär eller konjunktiva kommensala mikrobiom unik ögonvabb aerob /fakultet anaerob kommensal förstärkning mikrobiologisk klassificering matris assisterad laser desorptionstid för flygning massa spektrofotometri identifiering MALDI-TOF identifiering relativ kommensal överflöd
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