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Journal / Bioengineering / En effektiv metode for Adenovirusproduksjon
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Bioengineering
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JoVE Journal Bioengineering
En effektiv metode for Adenovirusproduksjon
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En effektiv metode for Adenovirusproduksjon

Article DOI: 10.3791/61691-v • 10:06 min • June 10th, 2021
June 10th, 2021 •
1, 1, 1, 1
1Institute of Cellular Biology and Pathology "Nicolae Simionescu"

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Her presenterer vi en protokoll for adenovirusproduksjon ved hjelp av pAdEasy-systemet. Teknologien inkluderer rekombinasjon av pAdTrack og pAdEasy-1 plasmider, adenovirusemballasje og forsterkning, rensing av adenoviralpartiklene fra cellelysat og kulturmedium, viral titrering og funksjonell testing av adenoviruset.

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Tilbaketrekning utgave 172 Adenovirus pAdTrack grønt fluorescerende protein rekombinant seleksjon titer adenovirusrensing
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