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JoVE Journal Neuroscience
Visualisering af Calcitonin Gene-Related Peptid Immunoreactive Innervation af Rat Cranial Dura Mater med immunfluorescens og neural sporing
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Visualisering af Calcitonin Gene-Related Peptid Immunoreactive Innervation af Rat Cranial Dura Mater med immunfluorescens og neural sporing

Article DOI: 10.3791/61742-v • 07:28 min • January 6th, 2021
January 6th, 2021 •
*1, *1, 1, 1, 1, 1, 1, 1, 1
1Institute of Acupuncture and Moxibustion, China Academy of Chinese Medical Sciences
* These authors contributed equally

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Her præsenterer vi en protokol til visualisering af rumlig korrelation af calcitonin genrelateret peptid (CGRP)-immunoreaktive nervefibre og blodkar i kraniedura mater ved hjælp af immunfluorescens og fluorescerende histokemi med henholdsvis CGRP og falloidin. Derudover var oprindelsen af disse nervefibre retrograd spores med en fluorescerende neurale sporstof.

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Neurovidenskab Problem 167 kranie dura mater mellemste meningeal arterie calcitonin genrelateret peptid neural sporingsteknik fluorogold
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