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JoVE Journal Cancer Research
Un modello tridimensionale di sferoidi per studiare le cellule staminali del cancro del colon
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Un modello tridimensionale di sferoidi per studiare le cellule staminali del cancro del colon

Article DOI: 10.3791/61783-v • 06:38 min • January 22nd, 2021
January 22nd, 2021 •
1,2, 1,2, 1, 1,2
1Département de la recherche, Centre de Recherche en Cancérologie de Lyon, INSERM U1052, CNRS UMR5286, Université de Lyon, Université Lyon 1, Centre Léon Bérard, 2UMR-S1113 - IRFAC INSERM, Université de Strasbourg

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Questo protocollo presenta un nuovo sistema di coltura robusto e riproducibile per generare e far crescere sferoidi tridimensionali dalle cellule adenocarcinoma del colon Caco2. I risultati forniscono la prima prova di concetto per l'appropriatezza di questo approccio allo studio della biologia delle cellule staminali tumorali, compresa la risposta alla chemioterapia.

Tags

Ricerca sul cancro Numero 167 Cellule Caco2 Cellule staminali tumorali Proliferazione cellulare Cancro del colon Colonsfere Crescita delle colonsfere
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