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Immunology and Infection

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JoVE Journal Immunology and Infection
T- en B-celreceptor immuunrepertoireanalyse met behulp van next-generation sequencing
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T- en B-celreceptor immuunrepertoireanalyse met behulp van next-generation sequencing

Article DOI: 10.3791/61792-v • 08:59 min • January 12th, 2021
January 12th, 2021 •
1,2, 3,4, 1, 3,4, 1,2
1Pediatric Gastroenterology Unit, Edmond and Lily Safra Children's Hospital, Sheba Medical Center, 2Sackler Faculty of Medicine, Tel Aviv University, 3Cancer Research Center, Sheba Medical Center, 4Molecular Hematology Laboratory, Sheba Medical Center

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Het huidige protocol beschrijft een methode voor DNA-isolatie van bloedmonsters en darmbiopten, generatie van TCRβ- en IGH PCR-bibliotheken voor de volgende generatie sequencing, prestaties van een NGS-run en basisgegevensanalyse.

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Deze maand in JoVE Next-generation sequencing TCRβ IGH immuunrepertoire adaptieve immuniteit T-cellen B-cellen
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