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JoVE Journal Cancer Research
To Flow cytometriske tilgange af NKG2D Ligand Surface Detection at skelne stamceller fra Bulk Subpopulations i akut myeloid leukæmi
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
Journal (Cancer Research)
Establishment of a Primary Culture of Patient-derived Soft Tissue Sarcoma
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Journal (Cancer Research)
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To Flow cytometriske tilgange af NKG2D Ligand Surface Detection at skelne stamceller fra Bulk Subpopulations i akut myeloid leukæmi

Article DOI: 10.3791/61803-v • 05:24 min • February 21st, 2021
February 21st, 2021 •
*1, *1, 2, 1, 3, 1, 1,2,3
1Department of Biomedicine, University Hospital Basel and University of Basel, 2Division for Hematology, University Hospital Basel and University of Basel, 3Department of Internal Medicine, Hematology and Oncology, University Hospital Tübingen
* These authors contributed equally

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Vi præsenterer to forskellige farvning protokoller for NKG2D ligand (NKG2DL) afsløring i humane primære akut myeloid leukæmi (AML) prøver. Den første tilgang er baseret på et fusionsprotein, der er i stand til at genkende alle kendte og potentielt endnu ukendte ligands, mens den anden protokol er afhængig af tilsætning af flere anti-NKG2DL-antistoffer.

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Kræftforskning Problem 168 NKG2DL CD34 AML LSC fusionsprotein FACS
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