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Journal / Immunology and Infection / Mikrofluidiske cokulturmodeller til dissekering…
JoVE Journal
Immunology and Infection

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JoVE Journal Immunology and Infection
Mikrofluidiske cokulturmodeller til dissekering af immunresponset i in vitro tumormikromiljøer
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
Journal (Immunology and Infection)
High Throughput Fluorometric Technique for Assessment of Macrophage Phagocytosis and Actin Polymerization
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Journal (Immunology and Infection)
Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
 
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Mikrofluidiske cokulturmodeller til dissekering af immunresponset i in vitro tumormikromiljøer

Article DOI: 10.3791/61895-v • 07:46 min • April 30th, 2021
April 30th, 2021 •
1, 1, 1, 2, 3, 3, 2, 3,4, 1
1CNR Institute for Photonics and Nanotechnology, 2Dept. of Oncology and Molecular Medicine, Istituto Superiore di Sanità, 3Istituto di Patologia Generale, Università Cattolica del Sacro Cuore, 4Tumor Immunology and Immunotherapy Unit, IRCCS Regina Elena National Cancer Institute

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I en tid med immunterapi og enkeltcellegenomisk profilering kræver kræftbiologi nye in vitro- og beregningsværktøjer til undersøgelse af tumor-immungrænsefladen i en ordentlig rumlig tidsmæssig sammenhæng. Vi beskriver protokoller til udnyttelse af tumorimmune mikrofluidiske co-kulturer i 2D- og 3D-indstillinger, kompatible med dynamisk, multiparametrisk overvågning af cellulære funktioner.

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Denne måned i JoVE udgave 170 Organer-på-chip tumorimmunologi kræft-immun krydstale tumormikromiljø immunstruktur cellulær dynamik og interaktioner mikrofluidisk immunkompetent tumor-on-chip immuno-onkologi cellesporing
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