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Journal / Bioengineering / Construyendo un sistema de iluminación simple y…
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Bioengineering

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JoVE Journal Bioengineering
Construyendo un sistema de iluminación simple y versátil para experimentos optogenéticos
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Construyendo un sistema de iluminación simple y versátil para experimentos optogenéticos

Article DOI: 10.3791/61914-v • 06:41 min • January 12th, 2021
January 12th, 2021 •
1, 2, 1, 1, 1, 3, 1, 1, 4, 1, 1
1Department of Bioengineering, University of California, San Diego, 2University of California, San Diego, 3Department of Mathematical Computational, and Systems biology, University of California, Irvine, 4Department of Biomedical Engineering, Duke University

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Este protocolo describe cómo realizar experimentos optogenéticos para controlar la expresión génica con luz roja y roja lejana utilizando PhyB y PIF3. Se incluyen instrucciones paso a paso para construir un sistema de iluminación simple y flexible, que permite el control de la expresión génica u otra optogenética con una computadora.

Tags

Bioingeniería Número 167 optogenética PhyB fitocromo LOV CRY2 nMag-pMag microcontrolador LED electrónica bricolaje LED iluminación
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