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Journal / Genetics / Sandfliege (Phlebotomus papatasi) Embryo…
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JoVE Journal Genetics
Sandfliege (Phlebotomus papatasi) Embryo Mikroinjektion für CRISPR/Cas9 Mutagenese
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Journal (Genetics)
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Journal (Genetics)
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Sandfliege (Phlebotomus papatasi) Embryo Mikroinjektion für CRISPR/Cas9 Mutagenese

Article DOI: 10.3791/61924-v • 05:44 min • November 17th, 2020
November 17th, 2020 •
1, 1, 1, 1, 2, 2
1Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 2University of Maryland Insect Transformation Facility, The Institute for Bioscience and Biotechnology Research

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Dieses Protokoll beschreibt die Schritte der gezielten Mutagenese von CRISPR/Cas9 bei Sandfliegen: Embryoentnahme, Injektion, Insektenzucht und -identifikation sowie Auswahl von Mutationen von Interesse.

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Dieser Monat in JoVE Ausgabe 165 Insekt Genombearbeitung Embryo-Injektion
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