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Journal / Developmental Biology / En 3D Tail Explant Culture för att studera…
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JoVE Journal Developmental Biology
En 3D Tail Explant Culture för att studera ryggradsdjur segmentering i zebrafisk
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En 3D Tail Explant Culture för att studera ryggradsdjur segmentering i zebrafisk

Article DOI: 10.3791/61981-v • 11:24 min • June 30th, 2021
June 30th, 2021 •
1,3, 1,2,3
1Division of Developmental Biology, Cincinnati Children’s Hospital Medical Center, 2Department of Pediatrics, University of Cincinnati College of Medicine, 3Department of Genetics, Albert Einstein College of Medicine

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Här presenterar vi protokollet för 3D-vävnadskulturen i zebrafiskens bakre kroppsaxel, vilket möjliggör levande studier av ryggradsdjur segmentering. Denna explant modell ger kontroll över axeln förlängning, förändring av morfogen källor och subcellulära upplösning vävnad nivå levande imaging.

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Utvecklingsbiologi utgåva 172 segmentering somiter zebrafisk ryggradsdjur vävnadskultur levande avbildning explant axelförlängning immunostaining
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