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Journal / Genetics / Reprogrammation directe des fibroblastes humains…
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Genetics
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JoVE Journal Genetics
Reprogrammation directe des fibroblastes humains en myoblastes pour étudier les thérapies pour les troubles neuromusculaires
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Reprogrammation directe des fibroblastes humains en myoblastes pour étudier les thérapies pour les troubles neuromusculaires

Article DOI: 10.3791/61991-v • 10:28 min • April 3rd, 2021
April 3rd, 2021 •
1, 1, 1, 2, 2,3,4,6, 5, 1,6, 1,6
1Center for Gene Therapy, The Research Institute at Nationwide Children’s Hospital, 2Center for Cardiovascular Research, The Research Institute at Nationwide Children’s Hospital, 3The Heart Center, Nationwide Children’s Hospital, 4Division of Genetic and Genomic Medicine, Nationwide Children’s Hospital, 5Department of Human Genetics, The University of Utah School of Medicine, 6Department of Pediatrics, The Ohio State University

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Ce protocole décrit la conversion des fibroblastes cutanés en myoblastes et leur différenciation en myotubes. Les lignées cellulaires sont dérivées de patients atteints de troubles neuromusculaires et peuvent être utilisées pour étudier les mécanismes pathologiques et tester des stratégies thérapeutiques.

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Génétique Numéro 170 Reprogrammation hTERT MyoD fibroblaste myoblaste trouble neuromusculaire test thérapeutique
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