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Journal / Biology / Generasjon murine primær kolon epitelial…
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Biology

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Generasjon murine primær kolon epitelial monolayers fra intestinale krypter

Article DOI: 10.3791/62156-v • 06:57 min • February 6th, 2021
February 6th, 2021 •
1, 1, 1, 1, 1
1Department of Pathology, School of Medicine, University of Michigan

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I denne protokollen beskriver vi hvordan du genererer murine primære epitelale kolonmonolayere direkte fra tarmkrypter. Vi tilbyr eksperimentelle tilnærminger for å generere samtidige monolayers på gjennomtrengelige filtre, konfluente monolayers for riper sår healing og biokjemiske studier, og sparsomme og konfluente monolayers for immunfluorescence analyse.

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Biologi Utgave 168 primærkultur 2D-enteroider 2D-kolonoider epitelceller epitelale monolayere tarmkrypter
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